5′-Degenerate 3′-Dideoxy-Terminated Competitors of PCR Primers Increase Specificity of Amplification
نویسندگان
چکیده
منابع مشابه
5'-degenerate 3'-dideoxy-terminated competitors of PCR primers increase specificity of amplification.
Amplification of a product in PCR with specific primers may be viewed as an artificial Darwinian-type "selection of the fittest". In other selective systems, such as general evolution, immune system and probably brain cortex, the stringency of selection is not absolute but rather degenerate, with selection of many highly fit units, not limited, however, to only the fittest. In PCR also, anneali...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98243st04